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germination medium

Discussion in 'Orchid Culture' started by jai, Feb 7, 2016.

  1. jai

    jai Orchid addict

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    I bought germination medium online from eBay but does not say how much water/type of water to use can anyone help?
     
  2. jai

    jai Orchid addict

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    1454864913805.jpg
    This is what I bought
     
  3. KellyW

    KellyW Orchid wonk Staff Member Supporting Member

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    You should ask the seller what the recommended mix is. I see on the label that it is called a "1 liter pre-pack" so I would guess that you would mix it with 1 liter of water... probably distilled. It looks like that is from AJ Orchids which is Troy Meyer
     
  4. Marni

    Marni Well-Known Member Staff Member Supporting Member

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  5. jai

    jai Orchid addict

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    The eBay sellers name was ajorchids, I was thinking the same thing about the 1 liter pre-pack but was not sure. Also I don't want to make all of it I only need it for five glass mason jars(like two cups of it).
     
    Last edited: Feb 7, 2016
  6. jai

    jai Orchid addict

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    Thank you Marni I will check it out:)
     
  7. Marni

    Marni Well-Known Member Staff Member Supporting Member

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    You will also need agar to make this gel. I used to use agar from the health food store, but it can vary from batch to batch so now I use tissue culture grade. If you didn't get some of that I can send you some when you are readyl. You can make a half batch if you have a good scale. Also, you can freeze the unused portion if you separate it before sterilizing/autoclaving/pressure cooker.
     
  8. jai

    jai Orchid addict

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    Thanks for the tips. Agar came mix in the medium, but thanks for the offer:) How long does it last frozen?
     
  9. jai

    jai Orchid addict

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    I have a few more questions. Is bleach or hydrogen peroxide better for sterilization? For containers is plastic okay?(I have glass jars but looking at cheap alternatives) Also for capping off containers is a screw on lid or a "cotton stopper"(opening stuffed with cotton balls) cover by foil better? I looked at some articles with many different ideas and opinions.
     
  10. Marni

    Marni Well-Known Member Staff Member Supporting Member

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    There are advantages and disadvantages to both bleach and hydrogen peroxide. In the end I would recommend you start with the bleach. For my flasking I use polypropylene deli containers. Most markets with sandwich or deli counters will be willing to sell you a few. You want to be sure that they are the kinds that can go in the microwave and have the number five on the bottom. You can use screw-on lids as long as you can find plastic and hopefully ones that are transparent so the light will get through. I would stay away with cotton stoppers. I don't use any kind of vents on my flasks and after six years haven't found a reason to start.

    Do you know how you are going to sterilize the media and flasks? I know some people do it in a microwave but I've never tried this. A pressure cooker is a much more certain thing.
     
  11. jai

    jai Orchid addict

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    I wa
    I was going to sterlize with pressure cooker, but might experiment with one microwaved.
     
  12. Marni

    Marni Well-Known Member Staff Member Supporting Member

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    Go with the pressure cooker if you have access to one. The microwave is a bit iffy from what I understand and you want to eliminate as many variables as possible.
     
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  13. jai

    jai Orchid addict

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    Would a tote with holes cut in the lid for your hands be a good sterile environment? I have read that some people use a plastic bag and throw it away after.
     
  14. KellyW

    KellyW Orchid wonk Staff Member Supporting Member

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    The only time I tried flasking (about 14-15 years ago) I used a dry cleaning bag with the top taped closed. I sterilized the inside with bleach, wore sterilized gloves, etc. It was very cumbersome because the wet plastic film would cling to everything. It would have worked better if I had some frame or objects inside to hold the top up like a tent. If I was going to do it again I wouldn't use a bag.

    For flasks I used containers that were sold as orchid flasks. They had a screw top but had a hole for a cotton plug. I didn't know better :confused:. Anyway, to make a long story short ... I achieved germination in 3 flasks and had little protocorms forming then eventually had some kind of contamination invade and take over, probably because of the cotton plugs. It was a fun experience, I learned a lot but did a lot wrong. For now I am content with letting others do the flasking.

    How ever you do it I think it will be a good experience and hopefully reward you with seedlings.

    Marni, thanks for clearing that up. ajorchids on Ebay ocassionally sells some of Troy's flasks so I assumed it was Troy's Ebay user name. Are Troy and Aaron partners in the flasking venture?
     
  15. Marni

    Marni Well-Known Member Staff Member Supporting Member

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    Kelly, I know that Aaron sells his own flasks on ebay sometimes. I don't think there in a partnership between them, at least I've never heard any mention of it.
     
  16. Marni

    Marni Well-Known Member Staff Member Supporting Member

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    By the way, I would recommend Aaron's book:
    Asymbiotic Technique of Orchid Seed Germination Paperback – 2009

    by Aaron J. Hicks (Author)
     
  17. naoki

    naoki Well-Known Member

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    Hicks' book is very detailed, and a great book. For a simpler book, I thought that this could be more approachable for some people to see the overall procedure; lots of figures, and not a lot of text you have to read (I started with this book, and then started to read Hicks):
    Growing Orchids from Seeds by Seaton and Ramsey
    This book has a DIY construction of the "shield" (not completely sterile, but it blocks some contamination from the air above). Lots of people seems to be ok with a globe box (make holes to a big tupperware, and attach rubber grubs).
     
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  18. jai

    jai Orchid addict

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    I'm still undecided of what my sterile station will be
     
  19. Dave The Scientist

    Dave The Scientist Active Member

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    Do you have access to a fume hood? Maybe get friendly with your school's chem or bio teachers
     
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