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Sowing/Flasking Masdevallia green pod or dry seed?

Discussion in 'Orchid Culture' started by AdeljeanHo, Dec 2, 2016.

  1. AdeljeanHo

    AdeljeanHo New Member

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    Hello Fellow Enthusiasts,

    I have decided to venture into sowing seeds at home (with some tools from work at the university).

    I recently posted a Masdevallia floribunda that I had selfed. There are 5 pods growing and they will be 30 days tomorrow. I will abort 2 or 3 of them to not overly tax the plant as the spikes are developing their second bloom at the moment. This will be mostly for practice as a get ready to do some hybridization when more of my plants come into bloom.

    There is tons of info out there on general sterile methods and I am familiar with those as well. I will ba constructing a laminar flow hood in the coming months (24" × 24" or 24" × 18"). What is lacking is information specific to Masdevallia (except for the links below).

    • Does anyone have any experience with this species and how long the pods take to mature?
    • Reading suggests green pod is easier and more successful, but how long to wait before harvest? (I see Marni suggested 90 to 120 is the more common range).
    • Or should I let them develop until they dehisce?
    • Any media recommendations specifically for Masdevallia?
    http://www.oocities.org/~marylois/archiv73.html
    https://www.orchidsforum.com/threads/masdevallia-dry-seed-sowing.10425/#post-84550

    20161130_212738.jpg 20161130_212742.jpg 20161130_212806.jpg 14695499_10100570677973911_8177407874824657126_n.jpg
     
    jai likes this.
  2. Marni

    Marni Well-Known Member Staff Member Supporting Member

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    I've only sown Masdevallia floribunda once. I cut it at just 90 days, sowed it green capsule and had good germination. I have been told that it is the number of "sun days" that count. So in the winter it can take longer than in the summer. But generally, 90 days is good for masdevallias. I have had a few open at 60 or 70 days, but that is rare. If you let it open in the greenhouse and then sow it, you will have a lot of problems with contamination. That is easier to deal with in large seed with longer sterilization times than it is in small seed. Longer times with small seed may damage the seed.

    Leave at least several capsules on to mature. With a strong plant and only 90 days, the plant shouldn't be that stressed. You can sow them at different times and judge the measure of maturity by the quality of the seed. If it shakes out freely once you open the capsule it is close to opening. If you have to scrape it to get it out and it doesn't spread out in the flask with water, it may germinate but you could leave the others on longer.
     
  3. AdeljeanHo

    AdeljeanHo New Member

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    Thank you very much, Marni. It is in a naturalistic terrarium/vivarium, so I would imagine that there are tons of contaminants in such a small and dense enclosure. Interesting point on the counting "sun" days. Since this is inside and under consistent conditions, I wonder if the plant "thinks" if it is in summer or winter.

    I'll start sowing on day 90 and take it from there and see how they all go.

    Do you have preferred media for Masdevallia?
     
  4. Marni

    Marni Well-Known Member Staff Member Supporting Member

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    1/2 strength P668 with some banana and 10grams of sugar should work fine.
     
  5. AdeljeanHo

    AdeljeanHo New Member

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    P668 is from Phytotech, correct?

    They list P668 as listed without agar, do you add agar yourself? Or use P658 (it this the same formulation but with agar)?

    P668.JPG
     
  6. Marni

    Marni Well-Known Member Staff Member Supporting Member

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    I add the agar to P668. O156 is the same thing with banana. It is easier to have the base and add what you want than to buy small quantities of very similar products.
     
  7. AdeljeanHo

    AdeljeanHo New Member

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    That makes sense. Thank you.
     
  8. AdeljeanHo

    AdeljeanHo New Member

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    When recipes mention coconut water. Is it water from mature, husked coconuts (see image) or from fresh (ripe) coconuts with still a wet husk (green/yellow/ whatever color they are once ripe depending on cultivar)?

    20170104_171930.jpg
     
  9. Marni

    Marni Well-Known Member Staff Member Supporting Member

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    I use the one you show there.
     
  10. AdeljeanHo

    AdeljeanHo New Member

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    That makes it easy! Thanks
     
  11. AdeljeanHo

    AdeljeanHo New Member

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    I just wanted to share an update.

    I have not been able to get the selfings to germinate yet. They are dehiscing at about 55-60 days in my vivarium. I am playing chicken and keep losing by 1 or 2 days.

    I have had some success with sterilizing dry seed using H2O2. This is a flask of my first hybrid using this plant as the seed parent.

    [​IMG]
     
  12. Marni

    Marni Well-Known Member Staff Member Supporting Member

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    Thanks for the update. Those look great! Well done.