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Update on my flasking operation

Discussion in 'Growing Areas' started by Marni, Dec 22, 2010.

  1. Alexey

    Alexey Well-Known Member Supporting Member

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    Quote: Are you flasking too?

    I am at the beginning of pollination/hybridization and seed propagation (at he next step of addiction). Recently searched Propagation archives on the forums. If I not messed something, using dali containers is quite unique method.

    How do you seal lids? Do you use vented flasks?

    Thanks
     
  2. Marni

    Marni Well-Known Member Staff Member Supporting Member

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    I think others use them too. I found them while trying to find polypropylene jars. I don't do anything special to seal the lids. You just have to be careful to get a brand that has a tight lid. For a while I was sealing them in bags, but the rate of contamination was the same. So far, I haven't used vented flasks.
     
  3. Ray

    Ray Orchid Iconoclast Supporting Member

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    I have heard of folks putting a hole in the lid, then putting a round, medicated band-aid over it, but I'm not certain what venting accomplishes, or should I say, if it's worth doing.
     
  4. Alexey

    Alexey Well-Known Member Supporting Member

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    Thanks Marni, your info us very helpful.

    Ray: they claim, that vented lids allow seedlings transfer metabolism from consuming readily available sugars of the flask media to producing hydrocarbons using atmosphere CO2 on earliest stages due to gas exchange. As a result, more vigorous growth and less stressful adaptation after deflasking.
     
  5. Marni

    Marni Well-Known Member Staff Member Supporting Member

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    Alexey, do you have experience with vented flasks? When I started, everyone I talked to said not to worry about them with mothers but were less certain about the benefits of vented flasks on replates. As I got beyond mothers, I never went back and examined this.
     
  6. xmpraedicta

    xmpraedicta Prairie angraecoid nut Supporting Member

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    I used vented flasks for replates, but was getting too much dessication (I didn't have my flasks in a humid environment) so I ended up using parafilm to cover the lids to keep in moisture. The plants didn't mind at all!
     
  7. Marni

    Marni Well-Known Member Staff Member Supporting Member

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    Calvin, is parafilm airtight? Are you saying you had vents but covered them up? Are you still flasking?
     
  8. Alexey

    Alexey Well-Known Member Supporting Member

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    Marni,
    My personal experience not significant enough to tell something for sure.
     
  9. Marni

    Marni Well-Known Member Staff Member Supporting Member

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    Thanks, Alexey. There are so many variables to work on that I think I'll wait on venting until I run into a problem that I can't figure out.
     
  10. xmpraedicta

    xmpraedicta Prairie angraecoid nut Supporting Member

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    Hi Marni - I'm not flasking anymore...I had my little adventure back in 2007, just playing around and experimenting, so I'm far from experienced. I was advantaged with a functioning autoclave and tissue culture hood, so I had it easy. The parafilm is air-tight...I used it when I saw the agar cracking and drying. I completely covered the top of the flask (if you haven't touched it before, the film stretches over things and seals them). I covered all my flasks and of the three or four species I did it for, there was no real negative impact. They were uncovered for about a month or two, and then covered for the rest of the duration until deflasking.
     
  11. Uluwehi

    Uluwehi angraecoids, dendrobiums and more Supporting Member

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    Marni, we are using the parafilm on our Angraecopsis parviflora flasks too. It's great stuff!
     
  12. Magnus A

    Magnus A Ph.D.

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    A comment, parafilm is not air tight but waterproof. Gases like oxygen and nitrogen diffuses through parafilm over time.
     
  13. Marni

    Marni Well-Known Member Staff Member Supporting Member

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    Thanks, Magnus, do you know if it stops mites from getting in?
     
  14. Magnus A

    Magnus A Ph.D.

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    Yes Marni, parafilm is a "plastic paraffin film" and mites should not be able to "get in".

    Though it is possibly that the film breaks down with time...

    http://en.wikipedia.org/wiki/Parafilm

    /Magnus
     
  15. Marni

    Marni Well-Known Member Staff Member Supporting Member

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    Thank you. Good to know.